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KMID : 0379120150430040231
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Korean Journal of Mycology
2015 Volume.43 No. 4 p.231 ~ p.238
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Genome-wide Single Nucleotide Polymorphism-based Assay for Phylogenetic Relationship of the Flammulina velutipes
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Woo Sung-I
Kim Eun-Seon
Han Jae-Gu
Jang Kab-Yeul
Shin Pyung-Gyun
Oh Youn-Lee
Oh Min-Ji
Jo Sung-Hwan
Lee Jeong-Hee
Kim Kyoung-Su
Kong Won-Sik
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Abstract
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Genome-wide reanalyzed data of 25 Flammulina strains were compared against the reference genome (KACC42780) to establish a genome-wide single nucleotide polymorphism (SNP). The rate of mapping differences between the strains reflected in the strain variation in its result. Genome-wide SNPs distribution divided into types of homozygous SNP and heterozygous SNP moreover all of the strains demonstrated a wide variation in all of the regions. In the further study of topological relationship between the collected strains, phylogenetic tree was separated into 3 major groups. Group I contained F. velutipes var. related strains of ASI 4062, 4148, 4195. Group 2 contained strains that are different species of ASI 4188 F. elastica, ASI 4190 F. fennae, and ASI 4194 F. rossica. The other 19 strains F. velutipes were classified as a single group. However, further experiment to discriminate its genetic relationship between the white group and brown group did not verify its validity. The inferred tree exhibited a phylogenetic relationship between Korea white fruitbody forming strains of ASI 4210, 4166, 4178 and Japan white fruitbody forming strains of ASI 4209, 4167 confirmed to be genetically closely related.
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KEYWORD
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Flammulina velutipes, SNP, Single nucleotide polymorphisms, Phylogenetic analysis
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